FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005317955

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005317955
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4217071
Sequences flagged as poor quality0
Sequence length35-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC103570.2455970032280699No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN89170.21145007992514234No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC71740.17011807484389047No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA52250.12390116267902533No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGGT49700.049.760989
TGGCGCG52250.047.7166415
CGCGGTG54700.045.4008110
CCGAACT8100.043.91915567
CTACTCG61400.041.34527637
TACTCGG61600.041.29512838
GTAGTCC63800.039.3881327
ACTCGGG65550.039.08912739
TAGTCCC64550.038.9462828
CGTGCCT64200.038.94259320
TGTAGTC66200.037.9443226
CGAACTT10000.037.37500868
GCGTGCC67550.037.0482619
GGCGCGT68300.036.75655416
AGGATCG66100.036.74460659
GCCGGTT9750.036.733023
CCTGTAG69600.036.0131624
GATCGCT67750.035.74813561
CGGTTCA9750.035.6734125
CGGGTAG6900.035.547341