FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005317958

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005317958
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3711292
Sequences flagged as poor quality0
Sequence length35-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG120510.3247117176444214No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC67330.18141930087958588No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG49590.13361923556540417No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG48490.13065530817839177No Hit
GGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTC47100.1269099817529852No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN41190.11098560824640044No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37670.1015010406079608No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA36400.036.09708432
GCGGCAC38550.034.1826133
CGGCACC38700.034.0584234
CCGTCGC36600.033.11628345
TCGCCGG36900.032.98000348
TGGCCGT14450.032.94510770
ACCCGCG41700.031.6125825
CCCGTCG38500.031.4627244
CCCCCGA42400.030.80882819
GCACCCC44650.029.53984336
CCCCGAC45250.029.0266120
GGCACCC46250.028.66024635
TCGCGTA854.388299E-628.4476726
ATGGCCG13200.028.33660969
CCCCCGT43200.028.18054442
GTTCCGT3750.027.7193681
CACCCCC48000.027.4148137
AGTCCGC50300.026.8519867
CAGTCCG48700.026.6702146
GATAGGC24300.026.59970340
Produced by FastQC (version 0.11.5)