FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005317960

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005317960
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3674315
Sequences flagged as poor quality0
Sequence length35-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG131180.35701892733747653No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC65200.17744804133559589No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG52180.14201286498299684No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG49090.13360313418963807No Hit
GGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTC44620.12143760129439092No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN42040.11441588432129525No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37520.1021142716397478No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA41150.036.39378432
CGGCACC43650.034.09396734
GCGGCAC43850.034.08548733
ACCCGCG46350.032.3934525
TCGCCGG43350.032.26725848
CCGTCGC42750.032.2641945
CCCCCGA47150.031.73190119
CCCGTCG44350.031.4768844
CCCCGAC49050.030.6507320
GCACCCC49800.030.31936636
GGCACCC51800.029.07097435
CACCCCC52000.028.7798737
GTTCCGT4250.028.464221
CCCCCGT49850.027.83909442
AGTCCGC55150.027.300867
CAGTCCG53050.026.9494746
TCCGCCC57800.026.8856189
GTCCGCC56350.026.8420418
CGTCGCC52700.026.4500246
TGGCCGT14900.026.16353470
Produced by FastQC (version 0.11.5)