FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005317961

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005317961
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3674315
Sequences flagged as poor quality0
Sequence length35-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC119700.32577500840292684No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC105870.28813533951226283No Hit
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG95330.2594497205601588No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA70260.19121931570918663No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN41830.11384434921883399No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT40000.10886382904024287No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG20350.058.8573074
TACGGCC20550.058.284497
TCTACGG21350.056.100525
CTACGGC21750.055.0687946
GCGCGGT61050.052.7510729
TGGCGCG64550.050.1047215
CGCGGTG64550.050.04988510
GAACGCG24550.048.02427324
ACGGCCA25350.047.656868
GCCGGTT9350.047.2541163
GCCATAC25450.047.0614411
TGAACGC25200.047.05288723
GGCGCGT70600.046.59330716
CCGAACT9400.046.40092567
CGCGTGC71200.046.21515318
CCCGATC25600.045.7336231
CTGAACG26500.044.99632622
GCCCGAT26250.044.85036530
GCGTGCC73600.044.76664719
ATCTCGT26200.044.74318735
Produced by FastQC (version 0.11.5)