FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005317962

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005317962
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3686399
Sequences flagged as poor quality0
Sequence length35-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG132230.35869692889999155No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC62110.16848420369037642No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG51680.14019101025146763No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG50830.13788523705654218No Hit
GGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTC43460.11789282711936498No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN39670.10761179134434445No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36870.1000163031728253No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA41950.034.9693832
GCGGCAC43550.033.53449633
CGGCACC43500.033.50380334
TGGCCGT13400.032.36278570
ACCCGCG47000.031.5996425
TCGCCGG43550.031.21924648
CCCCCGA47000.031.18934419
CCGTCGC43200.031.02916545
CCCGTCG43800.030.59155544
GCACCCC49200.030.34120636
GTTCCGT3900.030.1676941
GGCACCC50300.029.18818735
CCCCGAC51350.028.55263920
CACCCCC53050.028.08108937
CCCCCGT49000.027.32716642
AGTCCGC55200.026.8565397
CCGCGCG55600.026.78147927
CCCGCGC56350.026.36034426
GTCCGCC57800.025.7680348
CAGTCCG54750.025.6255826
Produced by FastQC (version 0.11.5)