FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005317963

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005317963
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3686399
Sequences flagged as poor quality0
Sequence length35-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC117060.3175456590564396No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC101190.2744955171700079No Hit
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG92950.2521430805509659No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA72820.19753694594643717No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN39650.10755753785740502No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT39580.10736765065311706No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG22000.059.4983144
TCTACGG22350.058.2575155
TACGGCC22750.056.4956257
CTACGGC23650.054.9166226
GCGCGGT56200.051.8256269
GAACGCG25300.050.85459524
TGAACGC25900.049.80055223
ATCTCGT25650.049.49767735
CGCGGTG59650.049.05985310
TGGCGCG61000.048.6352815
CGGCCAT26300.048.4756779
ACGGCCA26750.048.4351588
GCCATAC26100.048.3169711
CCTGGTT39200.047.48406670
TCGTCTG26750.047.3726438
CGTCTGA27050.047.1213139
CCCGATC26950.047.05356231
GCCCGAT27200.046.8598930
CTCGTCT27450.046.14821637
GGCGCGT65050.046.0351316
Produced by FastQC (version 0.11.5)