FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005317965

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005317965
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3684734
Sequences flagged as poor quality0
Sequence length35-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC113870.30903180528092394No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC101600.2757322509575996No Hit
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG93650.25415674510018904No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA72680.1972462598385664No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN39750.107877529287053No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT38360.10410520813714098No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG21150.060.4284024
TACGGCC21250.059.821377
TCTACGG22100.057.6760675
CTACGGC22750.055.8771066
GCGCGGT57450.052.4309849
ACGGCCA25300.050.5182958
GAACGCG25000.050.48782724
CGCGGTG60100.050.11913710
GCCATAC26000.049.29037511
TGGCGCG61600.049.12367215
TGAACGC26050.048.31283623
CCCGATC26350.048.258831
CGGCCAT26450.048.190599
ATCTCGT26150.048.01970335
GCCCGAT27250.046.52256830
CCATACC27800.046.34616512
CCTGGTT39950.046.11322470
GGGTCTA22150.045.3804742
TCGTCTG27550.045.36047738
CCGAACT9200.045.09065667
Produced by FastQC (version 0.11.5)