FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005317966

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005317966
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2984241
Sequences flagged as poor quality0
Sequence length35-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG184640.6187167859432264No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG75890.25430251779263136No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN65160.2183469766684393No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG46950.1573264357670845No Hit
TGGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCAC35240.11808697755978824No Hit
GGGGGCAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG32550.10907296026024708No Hit
AGGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCAC32190.10786662337257615No Hit
CGTTTGAATATTTGCTACTACCACCAAGATCTGCACCTGCGGCGGCTCCACCCGGGCCCGCGCCCTAGGCTTCA30510.10223705123011179No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA53800.037.7315432
CGCGCTA952.2009772E-1036.327128
GCGGCAC56300.036.00803833
TCGCCGG54550.034.9860548
CCCGTCG55700.034.7140744
CGGCACC58550.034.6453834
CCGTCGC55450.034.45892745
GCACCCC62650.032.745636
CACCCCC63900.031.90594337
ACGATCG553.328955E-431.2490356
CCCCCGA66200.030.76319
ACCCGCG66450.030.65269325
AGGAGGG88300.030.50092570
GGCACCC67200.030.4588335
TAGTCCG3300.029.1657666
CCCCCGT65800.029.12870242
GTTCTCG20600.029.03332313
CCCCGAC70200.028.96891220
GGCGATC6500.028.70918370
GTGCCGT5050.028.6768571