FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005317969

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005317969
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2977285
Sequences flagged as poor quality0
Sequence length35-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN64100.21529682244057924No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC50480.169550446127932No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC41730.14016125429711968No Hit
GGGGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCCCACCCCTCCTCCCCGCGCCCCCGCCCCGGCG36780.12353536863283159No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGGT25600.048.9778449
TGGCGCG25950.048.3172615
TACTCGG31650.040.44521738
CGCGGTG31400.040.14843410
CCGAACT5100.040.1343767
CTACTCG32500.039.57466537
CGCGAAA451.0269957E-438.1729282
GCCGGTT5350.037.8830573
CGGATTG3050.037.2783431
ACTCGGG35150.036.92962639
GTAGTCC35150.036.59502827
GTACGCA3350.036.16063765
CGGTTCA5700.036.1595655
CGGGTAG4100.036.1349221
TAGTCCC35700.036.0519528
AGGATCG35000.035.1436859
TGTAGTC36600.035.129226
CGTGCCT36300.034.87405420
GATCGCT36400.033.58560661
GCGGATT2500.033.0760571
Produced by FastQC (version 0.11.5)