FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005317970

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005317970
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3059133
Sequences flagged as poor quality0
Sequence length35-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG196800.6433195287684452No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG82910.271024502694064No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN66620.2177741209682613No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG51260.16756381628389483No Hit
TGGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCAC36900.12062241164408347No Hit
GGGGGCAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG33690.11012924250106157No Hit
AGGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCAC33290.10882168248323952No Hit
CGTTTGAATATTTGCTACTACCACCAAGATCTGCACCTGCGGCGGCTCCACCCGGGCCCGCGCCCTAGGCTTCA31650.10346068641016917No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA57800.037.5045232
GCGGCAC59600.036.4490733
TTATCGG2350.035.0979737
CGGCACC61850.035.02916334
CCGTCGC59500.034.69145245
CCCGTCG59650.034.63100444
TCGCCGG59850.034.40417548
AGGACCG8250.033.1891170
GCACCCC66700.032.8324136
CGCCCTA13800.032.11169461
AGGAGGG93150.031.62634870
CCCCCGA69100.031.30680519
CACCCCC69900.031.10255837
GGCACCC71850.030.41125535
ACCCGCG71400.030.37153425
CCCCCGT69800.029.74731842
GTTATCG2800.029.4572286
GTCGTAC5050.029.26279311
CCCCGAC74600.028.96585720
TATCGGA2650.028.5309018