FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005317971

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005317971
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3059133
Sequences flagged as poor quality0
Sequence length35-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN65740.21489748892905275No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC50640.16553709825627066No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC41170.13458061483433378No Hit
GGGGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCCCACCCCTCCTCCCCGCGCCCCCGCCCCGGCG36720.12003400963606355No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGGT26600.046.1226169
TGGCGCG27500.045.1011915
CCGAACT5850.040.4912167
CGCGGTG31200.039.65292410
GCCGGTT6000.038.3657883
GTACGCA4650.038.3113265
CGGTTCA6100.037.738085
CTACTCG34250.036.8419637
TACTCGG34550.036.53957738
CGGCATA2300.035.9649541
TCGCGTA400.00228928935.39855660
GTAGTCC35400.035.36186227
GGTCGTA1950.035.238962
TAGTCCC36200.034.69048328
GTCGTAC1700.034.357423
TGTAGTC36900.034.0072226
ACTCGGG37850.033.92774639
CGGCAAA5500.033.8397561
CGAACTT7150.033.8256768
CGGTAAG2650.033.816111
Produced by FastQC (version 0.11.5)