FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005317972

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005317972
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3029722
Sequences flagged as poor quality0
Sequence length35-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG189290.6247767947026163No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG82050.2708169264374751No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN65690.21681857279314737No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG49330.1628202191488196No Hit
TGGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCAC36830.12156230835700438No Hit
GGGGGCAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG32890.10855781487542422No Hit
AGGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCAC32890.10855781487542422No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA55750.036.03874232
GCGGCAC56850.035.357933
CGGCACC58250.034.3493634
TCGCCGG57250.033.52149248
CCGTCGC57400.033.4093445
CCCGTCG57800.033.2781244
CGCGCTA652.3169294E-531.8578728
GCACCCC65250.030.96523736
AGGAGGG91100.030.09170570
CCCCCGA67500.029.86389219
ACCCGCG68050.029.58609225
CACCCCC68950.029.42177637
AGGACCG7600.029.39073870
GGCACCC69200.029.03051835
CCCCCGT68300.028.0178242
CCCCGAC72100.027.96829620
GTTATCG2350.027.7870856
TATCGCG756.329597E-527.49458910
CGTACGT2400.027.23296719
TAGTCCG2950.026.7955746