FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005317975

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005317975
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3509585
Sequences flagged as poor quality0
Sequence length35-76
%GC62

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA369091.0516628034368736No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN276740.7885262787480571No Hit
GGGGACATTTGCTTCTGACACAACTGTGTTCACTAGCAACCTCAAACAGA82500.2350705282818339No Hit
CGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA79300.2259526411242355No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC66220.188683277367552No Hit
GGGGGGCCCTTCCCGTGGATCGCCCCAGCTGCGGCGGGCGTCGCGGCCGC64830.1847226951334702No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC52490.14956184278198134No Hit
GGGGGGCCACGGCAAGAAGGTGGCCGACGCGCTGACCAACGCCGTGGCGC51350.1463135954820869No Hit
GGGGGGCACCTTTGCCACACTGAGTGAGCTGCACTGTGACAAGCTGCACG47710.13594199884031874No Hit
GGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA47560.1355145978798063No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC45110.12853371552477003No Hit
GGGGGTTAAGGGCCACGGCAAGAAGGTGGCCGACGCGCTGACCAACGCCG42070.11987172272505155No Hit
GGGGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCCCACCCCTCCTCCCCGCGCCCCCGCCCCGGCG37480.10679325333337134No Hit
GGGGGGAAGGCCCGGCGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGT35890.10226280315193961No Hit
GGGGGACAACCTCAAGGGCACCTTTGCCACACTGAGTGAGCTGCACTGTG35390.10083813328356486No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGACT63900.055.7371831
ACTCTTC79900.054.6316385
GACTCTT81100.053.8650674
GGACTCT79500.053.8020133
TCTGGTC80300.053.3036210
TCTTCTG82000.052.4044387
CTCTTCT83600.052.1326526
TTCTGGT82300.052.0898789
GGGACTC78400.051.964552
GCGCGGT31100.051.215359
CTGGTCC84750.049.9856611
CGGGACT16550.049.7412381
TGGTCCC86600.048.91784312
CGCGGTG34600.046.2311610
CCGAACT5300.046.1097967
TGGCGCG35900.044.93465415
CGGTTCA5400.044.5594375
ACAGACT95950.043.82634720
GTCCCCA97600.043.7858914
CCCCACA97250.043.59624516