FastQCFastQC Report
Wed 8 May 2024
EGAF00005317977

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005317977
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3547500
Total Bases261.2 Mbp
Sequences flagged as poor quality0
Sequence length35-76
%GC62

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTG408311.1509795630725863No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN277120.781169837914024No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCT136450.3846370683579986No Hit
CGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTG91140.25691331923890065No Hit
GGGGACATTTGCTTCTGACACAACTGTGTTCACTAGCAACCTCAAACAGA90260.25443269908386185No Hit
GGGGGGCCCTTCCCGTGGATCGCCCCAGCTGCGGCGGGCGTCGCGGCCGC69500.19591261451726566No Hit
GGGGGGCCACGGCAAGAAGGTGGCCGACGCGCTGACCAACGCCGTGGCGC54510.15365750528541228No Hit
GGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGT53270.15016208597603944No Hit
GGGGGGCACCTTTGCCACACTGAGTGAGCTGCACTGTGACAAGCTGCACG49020.13818181818181818No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC47780.13468639887244538No Hit
GGGGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCCCACCCC45280.12763918252290346No Hit
GGGGGTTAAGGGCCACGGCAAGAAGGTGGCCGACGCGCTGACCAACGCCG42630.120169133192389No Hit
GGGGGGAAGGCCCGGCGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGT37460.10559548978153628No Hit
GGGGGACAACCTCAAGGGCACCTTTGCCACACTGAGTGAGCTGCACTGTG35750.10077519379844961No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)