FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005317977

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005317977
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3547500
Sequences flagged as poor quality0
Sequence length35-76
%GC62

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA383771.0818040873854828No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN277120.781169837914024No Hit
GGGGACATTTGCTTCTGACACAACTGTGTTCACTAGCAACCTCAAACAGA88040.24817477096546864No Hit
CGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA84680.23870331219168428No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC69400.195630725863284No Hit
GGGGGGCCCTTCCCGTGGATCGCCCCAGCTGCGGCGGGCGTCGCGGCCGC69190.1950387596899225No Hit
GGGGGGCCACGGCAAGAAGGTGGCCGACGCGCTGACCAACGCCGTGGCGC54310.1530937279774489No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC53600.151092318534179No Hit
GGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA49920.14071881606765327No Hit
GGGGGGCACCTTTGCCACACTGAGTGAGCTGCACTGTGACAAGCTGCACG48870.13775898520084567No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC47760.13463002114164907No Hit
GGGGGTTAAGGGCCACGGCAAGAAGGTGGCCGACGCGCTGACCAACGCCG42460.11968992248062016No Hit
GGGGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCCCACCCCTCCTCCCCGCGCCCCCGCCCCGGCG41140.11596899224806201No Hit
GGGGGGAAGGCCCGGCGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGT37460.10559548978153628No Hit
GGGGGACAACCTCAAGGGCACCTTTGCCACACTGAGTGAGCTGCACTGTG35630.10043692741367159No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGACT62300.056.284611
ACTCTTC78950.054.8580065
GGACTCT77900.054.334843
CGGGACT16600.052.860431
GACTCTT82650.052.730454
TCTGGTC80850.052.60025810
GGGACTC77550.052.311842
TCTTCTG82750.052.2158857
TTCTGGT82500.051.5519189
CTCTTCT84450.051.445896
GCGCGGT33100.050.3100439
CTGGTCC85250.050.0047511
TGGTCCC86250.049.30702212
CGCGGTG36350.046.08853510
GTCCCCA96900.044.41280714
TGGCGCG37650.044.31953415
CTACTCG38900.044.30595437
ACAGACT96700.043.66948320
TAGTCCC39200.043.6326328
CACAGAC98100.043.62692319
Produced by FastQC (version 0.11.5)