FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005317979

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005317979
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3640763
Sequences flagged as poor quality0
Sequence length35-76
%GC62

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA390361.072192834304238No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN286640.7873074957090038No Hit
GGGGACATTTGCTTCTGACACAACTGTGTTCACTAGCAACCTCAAACAGA87520.24038917117098807No Hit
CGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA84810.2329456764969321No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC70480.1935857950654849No Hit
GGGGGGCCCTTCCCGTGGATCGCCCCAGCTGCGGCGGGCGTCGCGGCCGC67870.18641696809157862No Hit
GGGGGGCCACGGCAAGAAGGTGGCCGACGCGCTGACCAACGCCGTGGCGC54630.15005096459176276No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC52490.1441730758085599No Hit
GGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA50980.14002559353629995No Hit
GGGGGGCACCTTTGCCACACTGAGTGAGCTGCACTGTGACAAGCTGCACG49560.13612531219417467No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC47750.13115382682146573No Hit
GGGGGTTAAGGGCCACGGCAAGAAGGTGGCCGACGCGCTGACCAACGCCG44340.12178765824636209No Hit
GGGGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCCCACCCCTCCTCCCCGCGCCCCCGCCCCGGCG39330.1080268064688638No Hit
GGGGGGAAGGCCCGGCGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGT38410.10549986362748688No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGACT67250.056.868451
ACTCTTC84500.055.3109475
GACTCTT85200.054.8159454
GGACTCT83900.054.169573
GGGACTC82700.053.1540152
TCTGGTC88300.052.86039710
TCTTCTG89250.052.3357437
TTCTGGT89800.051.9018759
CTCTTCT90300.051.837736
CGGGACT17350.051.576711
CTGGTCC93200.049.53531311
TGGTCCC94200.048.9367612
GCGCGGT31450.048.1046269
CCGAACT4400.046.3865167
CGTATCA2400.044.6464256
GTCCCCA104450.044.58478514
CCCCACA103450.044.4571616
CCACAGA104850.044.4447418
ACAGACT102550.044.31415620
GCCGTAT2500.044.15152454
Produced by FastQC (version 0.11.5)