FastQCFastQC Report
Wed 8 May 2024
EGAF00005317980

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005317980
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3596502
Total Bases264.8 Mbp
Sequences flagged as poor quality0
Sequence length35-76
%GC60

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC400851.1145551983566255No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG328980.9147221383444247No Hit
GGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCAC321950.8951753676210941No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN284460.7909351920282541No Hit
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG219370.6099537828701332No Hit
GGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCT194650.5412203301986208No Hit
GGGGGTACGGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGG157200.4370913737848609No Hit
GTGCGCGTGCAGGTCGCTCAGGGCGGACAGCGCGTTGGGCATGTCGTCCA144210.4009729453785929No Hit
GTGGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGT139480.387821277452369No Hit
GCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTT137990.3836783630316346No Hit
GGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTT137830.3832334863153142No Hit
GTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACG121200.3369941126127554No Hit
GGGGCTGGGGGGAGGCCCAAGGGGCAAGAAGCATGGCCACCGAGGCTCCA116250.32323073920159084No Hit
TGTCCAGGGAGGCGTGCACCGCAGGGGTGAACTCGGCGGGGAGGTGGGCG111210.3092171226374961No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG111100.30891126989502576No Hit
GTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCCT107610.29920739652028555No Hit
TGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGT106100.2950088725100111No Hit
GCCGGTGCAAGGAGGGGAGGAGGGCCCGTTGGGAGGCCCAGCGGGCAGGA101300.28166257102039705No Hit
GGGGTGAACTCGGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTG96030.2670094441765916No Hit
GTCCAGGGAGGCGTGCACCGCAGGGGTGAACTCGGCGGGGAGGTGGGCGG95640.2659250571805604No Hit
GGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTTGAAGTTG80980.22516322804769745No Hit
TGTGCGCGTGCAGGTCGCTCAGGGCGGACAGCGCGTTGGGCATGTCGTCC80850.22480176571568708No Hit
CCAGGGAGGCGTGCACCGCAGGGGTGAACTCGGCGGGGAGGTGGGCGGCC76720.21331838547566495No Hit
GGAGGTCAGCACGGTGCTCACAGAAGCCAGGAACTTGTCCAGGGAGGCGT74200.2063115771936176No Hit
GCACTGGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACC72040.20030574152329123No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGT67530.18776577908200803No Hit
GGTGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCT64160.17839556324450814No Hit
GGGGGGAGGCCCAAGGGGCAAGAAGCATGGCCACCGAGGCTCCAGCTTAA62210.17297362826435242No Hit
AGATGCTCAAGGCCCTTCATAATATCCCCCAGTTTAGTAGTTGGACTTAG61720.17161119332062097No Hit
GGGCATGTCGTCCACGTGCGCCACGGCGTTGGTCAGCGCGTCGGCCACCT59350.16502145696012405No Hit
GGGCCGGTGCAAGGAGGGGAGGAGGGCCCGTTGGGAGGCCCAGCGGGCAG58800.16349219324777242No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGGGTTTCGGTCCCGCCGCCGC52640.14636443966943435No Hit
TGCGGGAAGTAGGTCTTGGTGGTGGGGAAGGACAGGAACATCCTCTCCAG52520.146030782132194No Hit
GTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAGCA51330.14272201155456052No Hit
CGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCT50380.1400805560514077No Hit
ACGTGCAGCTTGTCACAGTGCAGCTCACTCAGTGTGGCAAAGGTGCCCTT48450.13471423066079208No Hit
GGTGCAAGGAGGGGAGGAGGGCCCGTTGGGAGGCCCAGCGGGCAGGAGGA47510.1321005799524093No Hit
ACGGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGA47480.13201716556809923No Hit
GGCTGGGGGGAGGCCCAAGGGGCAAGAAGCATGGCCACCGAGGCTCCAGC47410.13182253200470903No Hit
GGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAGC47390.13176692241516896No Hit
GGTGGTGGGGAAGGACAGGAACATCCTCTCCAGGGCCTCCGCACCATACT47230.1313220456988485No Hit
GCTGGGGGGAGGCCCAAGGGGCAAGAAGCATGGCCACCGAGGCTCCAGCT46910.13043229226620756No Hit
GCTTGTGCGCGTGCAGGTCGCTCAGGGCGGACAGCGCGTTGGGCATGTCG46370.12893083334862598No Hit
GTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTTGAAGTTGA45920.12767961758397467No Hit
CGCTCCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTA45630.12687327853564379No Hit
GGCAGTGGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGC45300.12595572030823285No Hit
GGCCGGTGCAAGGAGGGGAGGAGGGCCCGTTGGGAGGCCCAGCGGGCAGG45130.12548303879714234No Hit
GGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGTGC44760.12445426139065124No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44390.12342548398416016No Hit
CGTTTGAATATTTGCTACTACCACCAAGATCTGCACCTGCGGCGGCTCCA43710.12153475793979818No Hit
GCCTGCACTGGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACA42990.11953281271635606No Hit
GGACAGCAAGAAAGCGAGCTTAGTGATACTTGTGGGCCAGGGCATTAGCC41790.11619623734395255No Hit
CGGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGC41690.11591818939625226No Hit
GGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACGAG41610.11569575103809202No Hit
GCAGGCAGTGGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTG41540.11550111747470182No Hit
GGGGGCAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG41470.11530648391131161No Hit
TAGTGATACTTGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTG41150.11441673047867067No Hit
TGGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCAC40660.11305429553493923No Hit
GGGCTGGGGGGAGGCCCAAGGGGCAAGAAGCATGGCCACCGAGGCTCCAG40050.11135820305396744No Hit
TGGTGGTGGGGAAGGACAGGAACATCCTCTCCAGGGCCTCCGCACCATAC39790.11063527838994668No Hit
AGGGGTGAACTCGGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGT39260.10916162426713512No Hit
AGGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCAC38920.10821626124495412No Hit
AGTGGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCG37460.10415676120852985No Hit
CAGGTCGAAGTGCGGGAAGTAGGTCTTGGTGGTGGGGAAGGACAGGAACA37220.10348944613404913No Hit
TGCCATGAGCCTTCACCTTAGGGTTGCCCATAACAGCATCAGGAGTGGAC36650.10190457283215745No Hit
CCAGCTTAACGGTATTTGGAGGTCAGCACGGTGCTCACAGAAGCCAGGAA36340.10104262419428656No Hit
TAGGGAACAAAGGAACCTTTAATAGAAATTGGACAGCAAGAAAGCGAGCT36260.10082018583612631No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)