FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005317981

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005317981
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3596502
Sequences flagged as poor quality0
Sequence length35-76
%GC62

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA390671.0862499172807356No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN280000.7785342535608211No Hit
GGGGACATTTGCTTCTGACACAACTGTGTTCACTAGCAACCTCAAACAGA87250.24259683436850585No Hit
CGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA82780.23016809110630276No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC67620.1880160222349383No Hit
GGGGGGCCCTTCCCGTGGATCGCCCCAGCTGCGGCGGGCGTCGCGGCCGC67590.1879326078506282No Hit
GGGGGGCCACGGCAAGAAGGTGGCCGACGCGCTGACCAACGCCGTGGCGC53620.14908930955689723No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC53500.14875565201965688No Hit
GGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA53240.1480327273556361No Hit
GGGGGGCACCTTTGCCACACTGAGTGAGCTGCACTGTGACAAGCTGCACG49370.13727227177963475No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC47950.1333239909222906No Hit
GGGGGTTAAGGGCCACGGCAAGAAGGTGGCCGACGCGCTGACCAACGCCG44290.12314743603645988No Hit
GGGGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCCCACCCCTCCTCCCCGCGCCCCCGCCCCGGCG39280.10921723385667519No Hit
GGGGGGAAGGCCCGGCGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGT37940.10549139135749125No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGACT63600.056.1413841
ACTCTTC78850.054.688265
GACTCTT81150.053.2628334
GGGACTC76200.053.0302772
GGACTCT78850.052.9247673
TCTTCTG84650.050.9411627
TTCTGGT84050.050.9396639
TCTGGTC83900.050.90952310
CTCTTCT85400.050.6922656
CGGGACT15650.050.2414741
GCGCGGT31350.049.6324359
TGGTCCC87900.048.28429412
CTGGTCC87750.048.2509411
GTCCCCA96450.044.14462714
CCGAACT5050.044.03424567
CGCGGTG35550.043.959410
ACAGACT97300.043.61591720
GTACGCA4700.043.1892765
CCCCACA98550.043.06635716
CTACTCG38250.042.87407337