FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005317984

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005317984
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3755433
Sequences flagged as poor quality0
Sequence length35-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG172400.45906823527406826No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG76400.2034385915019653No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG45200.12035895727603182No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN42300.11263681178708287No Hit
CGTTTGAATATTTGCTACTACCACCAAGATCTGCACCTGCGGCGGCTCCACCCGGGCCCGCGCCCTAGGCTTCA41510.11053319284354161No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA51450.034.76740632
GCGGCAC53100.033.82960533
CCGTCGC52400.032.6959345
CGGCACC55350.032.5282734
TCGCCGG52950.032.29350348
CCCGTCG54150.032.06783344
GCACCCC60600.029.96525236
CCCCCGA61850.029.44092219
AGGACCG10400.029.41598370
CACCCCC63550.028.6414637
AGTCCGC65000.028.3636287
CAGTCCG63500.028.1110696
CCCCCGT61450.027.6611142
CGTACGT2500.027.58971419
AGGAGGG90900.027.37293670
ACCCGCG67150.026.99807225
GTCCGCC68850.026.8776728
GGCACCC67800.026.77269635
TCCGCCC70950.026.3735629
CCCCGAC69150.026.18783820
Produced by FastQC (version 0.11.5)