FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005317985

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005317985
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3755433
Sequences flagged as poor quality0
Sequence length35-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC42340.1127433241386546No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC41990.11181134106240213No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN41760.1111988950408648No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGGT25300.043.436759
TGGCGCG26850.041.0569815
CGGCAAT4900.036.6738741
TACTCGG30900.036.24690238
CTACTCG30950.036.05889537
CGCGGTG30750.035.96130810
CGGTAAG3250.035.0897371
CGGCAAA8050.034.772721
GGGGTAT14500.034.5580751
ACTCGGG33500.034.17203539
GGGTACG3450.034.057231
GGGGTAG18800.033.6389771
CCGAACT4900.033.5609167
CGGGTAG5150.033.5515251
GGGGGGA81550.033.350341
CGGCGAA3450.033.0555461
CGGCATA3050.032.8584941
CGGTATA1600.032.3981931
AGGATCG33350.032.3234359
GATCGCT33900.031.65261
Produced by FastQC (version 0.11.5)