FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005317986

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005317986
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3846716
Sequences flagged as poor quality0
Sequence length35-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG172750.44908436182967504No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG78570.20425214650626666No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG46460.1207783470367971No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN43150.11217360470593618No Hit
CGTTTGAATATTTGCTACTACCACCAAGATCTGCACCTGCGGCGGCTCCACCCGGGCCCGCGCCCTAGGCTTCA41700.10840415564861039No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA50800.036.5000332
GCGGCAC52550.035.294233
CGGCACC53900.034.1703834
CCGTCGC53300.032.46840745
TCGCCGG53800.032.17214648
CCCGTCG54400.031.9845644
GCACCCC60200.030.85751536
CCCCCGA62250.029.85443319
CACCCCC63150.029.48634337
ACCCGCG64700.028.92183725
AGGACCG9600.028.77378370
GGCACCC65650.028.12275935
CCCCCGT62800.027.73081442
AGTCCGC67650.027.6615647
GTACGTA3250.027.59212520
CAGTCCG66900.027.1474596
CGTACGT3200.026.93701619
CCCCGAC69650.026.78991320
GTCCGCC70950.026.423558
CGCCCTA18050.026.40806461