FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005317987

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005317987
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3846716
Sequences flagged as poor quality0
Sequence length35-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC43360.11271952491423855No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC42720.11105576808893612No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN42700.11100377568814541No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGTA505.8595106E-849.35936460
GCGCGGT24950.043.77739
TGGCGCG26250.041.8652715
CCGAACT5600.036.37988767
TACTCGG30900.036.25498638
CGCGGTG30350.036.21531710
CTACTCG30950.036.18077537
CGGCATA3300.034.5680961
GGGGTAC13750.034.190991
GGGGTAT15500.033.8990361
GCCGGTT6700.032.394793
CGGCATT6750.032.2635571
AGGATCG33900.032.106759
CGGGTAG6300.031.8245961
GGGGGGA81050.031.7318481
ACTCGGG35850.031.35772539
TAGTCCC35500.031.3281428
GTAGTCC35650.031.2821927
AAGTACG6450.031.27283563
TTAGCGG13050.031.15528910
Produced by FastQC (version 0.11.5)