FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005317988

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005317988
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3814055
Sequences flagged as poor quality0
Sequence length35-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG170710.44758137992241853No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG74690.19582832444734016No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG46020.12065898368009899No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN41490.10878186077547386No Hit
CGTTTGAATATTTGCTACTACCACCAAGATCTGCACCTGCGGCGGCTCCACCCGGGCCCGCGCCCTAGGCTTCA40840.10707763784213915No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA51450.035.71114332
GCGGCAC54750.033.63252633
CGGCACC56200.032.6540934
CCGTCGC53300.032.59009645
TCGCCGG53750.032.32448648
CCCGTCG54350.032.26148244
CGTACGT3050.030.52060719
CGATCTA3250.030.03213747
GCACCCC63200.029.05803336
CCCCCGA64500.028.49028219
CACCCCC64600.028.06912437
CCCCCGT63200.027.37946542
AGGAGGG94200.026.89981370
AGTCCGC68550.026.8952757
ACCCGCG68400.026.85139525
CGCCCTA18750.026.75057261
GGCACCC68950.026.47318535
CAGTCCG67050.026.3662416
GTCCGCC71550.025.7675918
TATCGGA2950.025.6998358
Produced by FastQC (version 0.11.5)