FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005317992

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005317992
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3569997
Sequences flagged as poor quality0
Sequence length35-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG200770.5623814249703851No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG139240.3900283389593885No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC106910.29946803876866No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG93010.2605324318199707No Hit
GGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCT86970.24361365009550429No Hit
GGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTC83170.2329693834476612No Hit
GTGGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGT81910.22943996871706054No Hit
GTGCGCGTGCAGGTCGCTCAGGGCGGACAGCGCGTTGGGCATGTCGTCCA75650.21190493997614002No Hit
GGGGGTACGGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGG67520.18913181159535988No Hit
GCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTT58740.16453795339323812No Hit
TGTCCAGGGAGGCGTGCACCGCAGGGGTGAACTCGGCGGGGAGGTGGGCG58440.16369761655261897No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG53510.14988808113844354No Hit
GCCGGTGCAAGGAGGGGAGGAGGGCCCGTTGGGAGGCCCAGCGGGCAGGAGGAACGGCTACCGAGGCTCCAGCTT50030.14014017378726087No Hit
GACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCGAAGTG48940.13708694993301115No Hit
GGGGCTGGGGGGAGGCCCAAGGGGCAAGAAGCATGGCCACCGAGGCTCCA48810.13672280396874284No Hit
GTCCAGGGAGGCGTGCACCGCAGGGGTGAACTCGGCGGGGAGGTGGGCGG48490.13582644467208235No Hit
GGGGTGAACTCGGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTG44450.12450990855174388No Hit
TGCGGGAAGTAGGTCTTGGTGGTGGGGAAGGACAGGAACATCCTCTCCAG43680.12235304399415461No Hit
TGGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCAC41220.11546228190107723No Hit
GGTGGTGGGGAAGGACAGGAACATCCTCTCCAGGGCCTCCGCACCATACT41000.11484603488462314No Hit
GGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTT40330.11296928260724028No Hit
AGGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCAC39660.11109253032985741No Hit
GGGCATGTCGTCCACGTGCGCCACGGCGTTGGTCAGCGCGTCGGCCACCTTCTTGCCGTGGCCCTTAACCTGGGC39280.11002810366507311No Hit
CCAGGGAGGCGTGCACCGCAGGGGTGAACTCGGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTT39200.109804013840908No Hit
GGAGGTCAGCACGGTGCTCACAGAAGCCAGGAACTTGTCCAGGGAGGCGT38900.1089636770002888No Hit
GGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTTGAAGTTG36580.10246507209950037No Hit
TGTGCGCGTGCAGGTCGCTCAGGGCGGACAGCGCGTTGGGCATGTCGTCC35700.10000008403368407No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA59950.036.3751932
GCGGCAC61500.035.63907233
CGGCACC62800.034.57776634
GTTCCGT3900.033.0700071
TCGCCGG62600.032.30666748
CCGTCGC63000.032.22380445
GCACCCC68250.032.03976436
CCCGTCG64250.031.79973644
CTTACCC15450.031.513670
ACGATCG553.238774E-431.395956
GATAGGC30500.031.21926140
GGCACCC70900.030.87943835
CCCCCGA72200.030.72784619
CACCCCC71250.030.70499837
GTTATCG2300.030.0309096
CCCCGAC75100.029.50106420
ACCCGCG74800.029.46183425
CATTAGC32600.029.13519513
TTCCGTT5000.029.0122972
AGTCCGC76550.028.693157
Produced by FastQC (version 0.11.5)