FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005317997

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005317997
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3576372
Sequences flagged as poor quality0
Sequence length35-76
%GC59

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA241780.6760482410666452No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC139920.39123446889753083No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC101790.2846180430894773No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT64550.18049017272252438No Hit
CGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA48190.13474549068161812No Hit
GGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA40890.11433374380517462No Hit
GGGCGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC37130.10382029609895167No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGGT61450.052.8501639
CCGAACT11350.051.66422767
GCCGGTT11950.050.5359083
GGGGACT53800.049.7443351
TGGCGCG66000.049.09730515
CGCGGTG66450.049.08122610
GTCTACG6600.048.6260454
TACGGCC6750.048.062097
TCTACGG6750.048.062095
CGGTTCA12500.048.0416375
TCTGGTC57350.047.30071610
ACTCTTC58200.047.20295
GGACTCT60650.046.9410823
GGGACTC60700.046.177572
CCGGTTC13050.046.0117384
TTCTGGT60950.044.5069059
CTGGTCC61750.044.09797311
CGGGACT13750.043.280571
CTTAGGC14200.042.78166218
CTACTCG76150.042.3766737