FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005317998

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005317998
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3678199
Sequences flagged as poor quality0
Sequence length35-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG133500.36294936733983124No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG59100.16067646149650958No Hit
GACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCGAAGTG42650.11595348701905471No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG36870.10023927471025902No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA44750.035.6015332
GCGGCAC48100.033.20625333
CGTACGT2300.033.0249719
CGGCACC49050.032.5715734
TCGCCGG46600.031.05062548
CCCGTCG45900.030.84479344
CCGTCGC46900.030.79949645
CCCCCGA55450.028.82883319
ACCCGCG55200.028.80509425
GCACCCC57350.028.17364536
AGTCCGC57150.027.8383267
CACCCCC58300.027.7240337
CAGTCCG55150.027.6589186
CCCCGAC59150.026.97117420
TCCGCCC60050.026.8943929
GTCCGCC59750.026.6262268
GTACGTA3000.026.4739820
CGATCTA2900.026.43335347
GGCACCC61450.026.28740135
CGCCCTA12950.025.57397561
Produced by FastQC (version 0.11.5)