FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318000

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318000
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3679892
Sequences flagged as poor quality0
Sequence length35-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG140370.3814514121610091No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG62580.17005933869798354No Hit
GACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCGAAGTG41290.11220437991114957No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG39410.10709553432546391No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA46500.035.14434432
GCGGCAC48550.033.66911733
CGGCACC50500.032.51327534
TCGCCGG48500.031.62201948
CCGTCGC49250.031.15477245
CCCGTCG48900.030.08136744
CCCCCGA56950.029.11243619
AGTCCGC57550.028.7959887
GCACCCC57500.028.69716336
GTTCCGT3500.028.6369671
CAGTCCG55700.028.63636
TTATCGG2450.028.1838267
ACCCGCG58800.028.16188225
GTCCGCC61150.027.1007218
CGTACGT2550.027.09073819
CACCCCC60850.027.0725237
TCCGCCC62100.027.0197129
CCCCGAC62600.026.37775220
AGGAGGG90850.025.57359570
GGCACCC65150.025.36927835
Produced by FastQC (version 0.11.5)