FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318001

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318001
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3679892
Sequences flagged as poor quality0
Sequence length35-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC89120.24218102052995036No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC74000.20109285815996772No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT47420.1288624774857523No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGGT44100.052.353869
TGGCGCG47750.048.2790115
CGCGGTG49550.046.59356310
CCGAACT9550.046.24389667
CGGTTCA10400.041.8117525
GCCGGTT10600.041.6740073
CGAACTT11450.040.6014668
CTACTCG56900.040.52076337
TACTCGG57850.039.9883238
CGGGTAG5450.038.7481
GTAGTCC60300.038.4096127
TAGTCCC60750.038.30415728
TGTAGTC61800.037.91811426
TACGGCC4750.037.0542267
ACTCGGG62950.036.98010639
CGTGCCT63000.036.941720
GTGGCGC62950.036.78495814
CCTGTAG64900.035.98913624
CCGGTTC12700.035.3265154
AGGATCG64450.035.0589759
Produced by FastQC (version 0.11.5)