FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318004

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318004
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3744140
Sequences flagged as poor quality0
Sequence length35-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG140190.37442510162547343No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG62820.16778218763187275No Hit
GACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCGAAGTG41170.1099584951417415No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG37950.10135838937646563No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA46700.033.8176532
TCGCCGG47650.031.910248
CCCGTCG46550.031.55190344
GCGGCAC50000.031.52360533
CCGTCGC49100.030.92023345
CGGCACC51100.030.85246734
AGTCCGC57100.028.2893037
GCACCCC56250.027.9849336
CAGTCCG55300.027.8369876
CCCCCGA57400.027.79180519
GTGCCGT6250.027.6588821
GCGAGAT5800.027.42374626
CGCGAGA5700.027.29348825
ACCCGCG58700.026.79752325
CGTACGT2450.026.77775819
CACCCCC59350.026.65186137
TCCGCCC62350.026.4054999
GTCCGCC61450.026.399068
CGAGATT6100.026.08202627
GTACGGC6100.026.02554714
Produced by FastQC (version 0.11.5)