FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318005

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318005
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3744140
Sequences flagged as poor quality0
Sequence length35-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC89140.23807870432195377No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC72160.19272783603177232No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT47140.12590341173139893No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGGT45750.050.445449
TGGCGCG48600.047.77178615
CGCGGTG51200.045.27849610
CTACTCG57750.040.2895537
TACTCGG57700.040.21183838
GCCGGTT8650.039.88093
GTAGTCC60150.039.08371427
TAGTCCC60450.038.90017328
CCGAACT8700.038.85700667
TGTAGTC61100.038.46417626
CGGTTCA9000.038.3304865
CGGTAAG3200.037.8840031
CGTGCCT61700.037.8204920
ACTCGGG62100.037.71196739
GTGGCGC64900.036.25203314
CCTGTAG64900.036.14668324
CGAACTT10050.035.79606668
CGGGTAG6000.035.7913631
GTCGTAC1850.035.429333
CGGCATA3650.035.1112821