FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318007

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318007
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4325450
Sequences flagged as poor quality0
Sequence length35-76
%GC61

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA221320.51166930608376No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN174760.40402732663653496No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC155000.3583442185206163No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC116970.27042273058294514No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT72460.16752014241292812No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC71970.16638731230276618No Hit
CGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA47760.11041625726802991No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGGT80500.052.7261289
CCGAACT11950.052.3853367
TGGCGCG87750.048.95779415
GGGGACT52650.048.7686351
ACTCTTC56650.048.5199935
CGCGGTG88100.048.3719410
GCCGGTT12800.047.6086353
CGGTTCA13050.046.95135
GGACTCT61650.046.924463
TCTGGTC58650.046.1645410
TACGGCC7450.045.944527
GTCTACG7600.045.9400754
TCTACGG7650.045.1923645
GTAGTCC95700.044.57186527
CTACTCG96850.044.0621737
TGTAGTC97200.043.8563626
TAGTCCC97250.043.8187628
CGTGCCT98150.043.76972620
GGGACTC62700.043.4120752
TACTCGG98100.043.35794438