FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318015

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318015
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3045075
Sequences flagged as poor quality0
Sequence length35-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC46520.1527712782115383No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC38640.1268934262702889No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGGT23650.045.906219
TGGCGCG25150.043.1661415
CGGTATA1550.040.36091
CGCGGTG27550.039.40769610
CGGCATA3150.038.616911
CCGAACT4750.035.7801667
CGGCAAA5950.035.0472831
CTACTCG31250.034.863337
TACTCGG32050.034.10864638
GGGGGGA53200.033.775281
GGGGTAT11650.033.4127461
CGGGTAG5100.033.392271
GGGGTAG13950.033.384941
CGGATAA2500.033.3650131
CGGTATG3150.033.100211
CGGTAAG3150.033.100211
CGGCAAT4100.033.0598451
AGGATCG33450.032.77527659
CGGCAAC5200.032.750111
CGGTACG851.1337579E-732.7107961
Produced by FastQC (version 0.11.5)