FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318018

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318018
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3034858
Sequences flagged as poor quality0
Sequence length35-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG124840.41135367783270255No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG45670.15048480027731118No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG33060.108934256561592No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA36300.035.3421132
GCGGCAC38000.033.8589833
TCGCCGG37200.033.15353848
CGGCACC39200.032.91584434
CCCGTCG38400.032.44214644
CCGTCGC38100.032.15172645
AGTCCGC44300.029.7553467
CCCCCGA44350.029.18728319
CCCCCGT42600.029.05904642
CAGTCCG44250.028.694366
ACCCGCG45400.028.52966325
GCACCCC45700.028.3203636
TCCGCCC48000.028.038339
GTCCGCC47150.027.8833968
CTCGTTT12900.027.624316
CACCCCC47150.027.53303537
CCCCGAC47050.027.44059620
CGCCCTA9350.027.3812761
GTTCTCG13400.026.85173213
TCGTTTG13100.026.6796317