FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318019

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318019
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3034858
Sequences flagged as poor quality0
Sequence length35-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC47310.1558886774933127No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC38350.12636505563027992No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGGT24650.047.841079
TGGCGCG25650.047.05298215
CGCGGTG28150.042.87562610
CGATAGG758.5310603E-1041.5017242
CTACTCG29400.040.2443837
TACTCGG30000.039.44928438
CGGCAAT4400.038.771911
CGGGTAG4100.037.3630831
CGGTATA2150.037.244591
GATCGCT31650.036.2238161
AGGATCG31200.036.02985859
GTAGTCC33200.035.9817227
CCGAACT5700.035.38426667
ACTCGGG33800.035.33331339
CGGCAAC5600.034.8155981
TAGTCCC34800.034.53494328
TGTAGTC34700.034.32029326
CGTGCCT35200.034.0087220
GGGGTAG14850.033.9950261
GGGGGGA56050.033.8528061
Produced by FastQC (version 0.11.5)