FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318020

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318020
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3034803
Sequences flagged as poor quality0
Sequence length35-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG121880.4016076167052688No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG47650.1570118389892194No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG33550.11055083311832761No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA36650.038.12492432
GCGGCAC38250.036.80756433
CGGCACC40100.034.94401634
TATCGCG601.3188726E-534.5924710
TCGCCGG38350.033.16154548
CCGTCGC39300.032.3314845
ACCCGCG43400.032.31348825
CCCGTCG40650.032.10239844
CCCCCGA44350.031.5978519
CCCCGAC45700.030.97086720
CGTACGT1700.030.5308219
GCACCCC46550.030.34423836
CACCCCC46300.030.29473337
AGTCCGC47350.030.246087
CAGTCCG45450.029.5315726
CCCCCGT44150.028.82801442
GTCCGCC50250.028.6377458
TCCGCCC51050.028.5955379
TAGTCCG3400.028.4883866
CCGCGCG49900.028.3875127
Produced by FastQC (version 0.11.5)