FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318021

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318021
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3034803
Sequences flagged as poor quality0
Sequence length35-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC46150.15206917879018836No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC37600.12389601565571141No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGGT24550.046.6252569
TGGCGCG26450.043.66246415
CGGTATA1950.042.8980261
CGCGGTG28750.040.29504810
CGGGTAG4500.039.5019341
CGGCATA2500.039.0372051
TACTCGG31400.037.14109438
CGGCATT6100.037.1401981
CTACTCG31500.037.01705637
CGGCAAT4050.037.006171
GTAGTCC33200.035.45345727
GGGGGGA56850.034.210891
TAGTCCC34900.033.73095328
ACTCGGG35250.033.6931939
CGTGCCT34600.033.58930620
GGGGTAG15250.033.3690381
TGTAGTC35650.033.204626
AGGATCG34050.033.11120259
GGTCGTA851.18334356E-732.5516552
GATCGCT35050.032.31065861
Produced by FastQC (version 0.11.5)