FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318023

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318023
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3016241
Sequences flagged as poor quality0
Sequence length35-76
%GC62

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN167180.5542660550002471No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA137190.4548376605185063No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC103220.3422140339581618No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC68020.2255124839162388No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC58310.19332009610637876No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT47230.15658563092272798No Hit
GGGGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCCCACCCCTCCTCCCCGCGCCCCCGCCCCGGCG42370.1404728600930761No Hit
GGGGGGAAGGCCCGGCGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGT36390.1206468581257267No Hit
CGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA31060.10297585637221958No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGGT45500.051.7442749
CCGAACT6700.051.4804667
TGGCGCG48500.048.6792315
CGCGGTG50150.046.87851310
ACTCTTC34700.046.6401335
GGGGACT34350.046.352141
TCTGGTC34900.046.2752310
CGGTTCA7350.045.4291275
GGACTCT38100.044.3515623
TACGGCC3650.043.8733377
CTACTCG55450.043.31335437
GCCGGTT7800.043.240713
TTCTGGT37600.042.9522749
GGGACTC38350.042.818742
TACTCGG56300.042.70838
TGTAGTC55550.042.49913826
GTAGTCC55600.042.44551527
TAGTCCC56650.041.57174328
CCTGTAG56850.041.46465324
TCTACGG3900.041.0609445