FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318027

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318027
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3011695
Sequences flagged as poor quality0
Sequence length35-76
%GC62

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN162900.5408914249284871No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA135910.45127411640288945No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC103310.3430294236302149No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC69590.2310658947868227No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC58190.19321345620987518No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT46670.15496257091106505No Hit
GGGGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCCCACCCCTCCTCCCCGCGCCCCCGCCCCGGCG42760.1419798485570418No Hit
GGGGGGAAGGCCCGGCGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGT35600.11820586081923966No Hit
CGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA30900.10260003087962095No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGGT48000.050.7839859
CCGAACT7700.050.5038467
TGGCGCG50850.048.27002715
CGGTTCA8000.047.663985
ACTCTTC33350.047.4700165
CGCGGTG52000.047.00846510
TCTGGTC34300.046.05521810
GGACTCT36350.045.1452563
GGGGACT32850.044.9531941
GCCGGTT8650.044.4766243
TTCTGGT35750.044.377719
CGGGACT8300.044.1696931
CTACTCG57050.043.743137
TACTCGG57600.043.6787338
GGGACTC36750.043.6355362
GTAGTCC57250.043.60040727
CCGGTTC8800.043.330894
TAGTCCC57900.043.15525428
TGTAGTC58450.042.84255626
CTGGTCC37000.042.50969711