FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318031

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318031
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3173582
Sequences flagged as poor quality0
Sequence length35-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC54830.17277007495000918No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC53450.16842167620058343No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGGT32000.048.1268429
TGGCGCG33250.045.79064615
CGGCATA2850.045.1447071
CGCGGTG35400.043.50449410
CGGGTAG5300.040.678581
GCCGGTT6150.040.5110173
CCGAACT5850.040.49654867
CGGCAAT3900.039.231781
CGAACTT6400.038.9257568
CTACTCG40250.038.02270537
TACTCGG40550.037.66475338
CGGTTCA6550.037.5129055
CGGTAAG2400.036.2225271
ACTCGGG42850.035.81301539
GTGGCGC43050.035.5275214
CGTGCCT43150.035.3789320
GTAGTCC44500.035.2608127
TAGTCCC44800.034.95233528
GCGTGCC43950.034.89082319
GGGGGGA62350.034.8572851
Produced by FastQC (version 0.11.5)