FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318034

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318034
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3160115
Sequences flagged as poor quality0
Sequence length35-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG100440.3178365344299179No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG39240.1241726962468138No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA31050.034.74921832
GCGGCAC33300.032.30377633
CGGCACC34050.031.5967534
TCGCCGG31800.031.43196348
CGTACGT1550.031.2793619
CCCGTCG33400.030.3109944
CCGTCGC33750.029.5852745
CCCCCGA37750.028.43847319
ACCCGCG38650.027.97273425
TTATCGG1404.4929038E-1027.2020997
GCACCCC40450.026.77548236
CCCCCGT38050.026.22517442
AGTCCGC41400.026.0910767
CCCCGAC41250.025.94566720
CGCCCTA8850.025.73121661
CGATCTA1900.025.65182747
CAGTCCG40150.025.6099456
CACCCCC42600.025.35069737
AACGGCG4400.025.33643249
TCGCCGT3350.024.894431
Produced by FastQC (version 0.11.5)