FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318035

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318035
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3160115
Sequences flagged as poor quality0
Sequence length35-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC55090.17432909878279745No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC54170.17141781232645015No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGGT33150.049.373879
TGGCGCG34250.047.8853815
CGCGGTG36800.044.7588310
CCGAACT5150.040.4503367
TACTCGG41250.040.31343538
CGGCATA2600.040.189851
CTACTCG41450.040.1137
CGGGTAG4400.039.580911
ACTCGGG44400.037.77992239
AGGATCG42300.037.7168259
GTGGCGC45050.037.09689314
GCCGGTT5750.036.7109263
CGTGCCT44800.036.54950720
GTAGTCC45400.036.47950427
CGCGTGC45150.036.41087318
CGAACTT5750.036.37632868
GCGTGCC45500.036.2097219
GATCGCT44500.036.1227361
GGCGCGT46000.035.95580716
GGGGGGA57000.035.9311371
Produced by FastQC (version 0.11.5)