FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318036

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318036
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3156834
Sequences flagged as poor quality0
Sequence length35-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG96640.3061294955642267No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG37680.11936009305525727No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA30250.034.62398532
TCGCCGG29650.033.83097548
GCGGCAC31950.032.89129633
CCGTCGC31500.031.9195445
CGGCACC33600.031.384834
CCCGTCG32600.031.36811444
GTTCCGT3650.029.7061881
CCCCCGA35900.029.23707219
ACCCGCG37250.028.28481925
AGTCCGC38700.028.0969927
CGCGCTA500.007557325527.73316228
CCCCCGT36700.027.4698142
AATCGCG2300.027.27348750
CAGTCCG37550.027.2052786
CCCCGAC39000.027.09375220
GCACCCC39850.026.82769436
GTCCGCC41150.026.6766058
GTTCGAC2800.025.97264317
TCCGCCC43100.025.7106979
CGTCGCC39400.025.61425846
Produced by FastQC (version 0.11.5)