FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318037

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318037
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3156834
Sequences flagged as poor quality0
Sequence length35-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC54710.17330654700247147No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC52190.165323865619795No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGGT32450.046.296159
TGGCGCG33800.044.8531415
CGGCAAT4450.043.932281
CGCGGTG35550.042.84261710
CCGAACT5550.040.09551667
CGGGTAG4450.038.4407461
TACTCGG40050.037.77776738
CTACTCG40050.037.76876437
CGGCAAA6300.037.681151
ACTCGGG42000.036.36874439
CGGCATT6750.036.2034531
GGGGTAG16200.036.2034531
CGTGCCT42450.035.8080520
GCCGGTT6650.035.3934333
GGGGTAT13150.035.3086931
AGGATCG42650.035.26067759
TAGTCCC43150.035.16810628
GCGTGCC43050.035.1436619
GTAGTCC43450.034.9219627
GGGGGGA59800.034.9104731
Produced by FastQC (version 0.11.5)