FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318038

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318038
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3397831
Sequences flagged as poor quality0
Sequence length35-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN136060.40043192260003513No Hit
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG132320.3894249007675779No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG52700.1550989440028065No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC46380.1364988429383333No Hit
GGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTC40770.11998831018964745No Hit
CGCTCCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTA35190.10356606905993852No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34720.10218283369596663No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG34470.10144707020449222No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCCGT13800.040.4359670
CGCGGCA39500.036.40723832
GCGGCAC42100.034.43798433
TCGCCGG40250.033.8224548
CCCGTCG42100.032.4270744
CGGCACC44400.032.374534
ATGGCCG13550.032.3703969
CCGTCGC42300.032.13976745
AGGACCG6700.031.91332870
CCCCCGA48550.029.78359819
ACCCGCG49300.029.43070225
GACGTAT3350.029.18275344
GCACCCC49550.029.12906536
CCCCGAC50800.028.48046320
CACCCCC51000.028.13143737
GGCACCC53150.027.06498535
CGTCGCC51000.026.75721546
CCGCGCG56150.025.812227
GTGCCGT6150.025.6123071
CAGTCCG55550.025.4273416