FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318039

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318039
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3397831
Sequences flagged as poor quality0
Sequence length35-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN135060.39748886863413746No Hit
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG105100.3093149718158437No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC70390.20716156865953606No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC58860.17322815643273606No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA41030.12075350422078085No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT34170.10056415401472293No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTACGG21000.059.1231845
GTCTACG20950.059.1014754
TACGGCC21350.057.994197
CTACGGC22700.054.6954546
GCGCGGT36600.051.723479
ACGGCCA24050.051.6252338
CGGCCAT25950.047.9767959
GCCATAC25950.047.57824311
TGGCGCG40050.047.26579315
GGGTCTA20750.046.5184022
CCATACC27500.045.0239112
CATACCA28100.044.18393313
CGCGGTG44800.042.56077610
CTACTCG45550.042.29149637
GGCCATA29550.042.24733710
GAACGCG29200.042.01290524
TGAACGC29600.042.00654623
TACTCGG46150.041.7767838
CGGTATA1400.041.621051
GTAGTCC46300.041.5305127
Produced by FastQC (version 0.11.5)