FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318041

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318041
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3383587
Sequences flagged as poor quality0
Sequence length35-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN133430.39434481808802313No Hit
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG104570.30905072043367No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC71310.21075267164698294No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC58270.17221368920024815No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA38870.11487808648041266No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT34490.10193324421686216No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG23500.060.9314424
TACGGCC24600.057.9296887
TCTACGG24550.057.769935
CTACGGC25550.055.508886
ACGGCCA28000.051.0163738
GCCATAC28000.050.651111
GCGCGGT38700.050.036979
CGGCCAT29150.049.237639
CCTGGTT44800.046.9812470
TGGCGCG41550.046.27593615
CATACCA30950.046.2632113
CCATACC30750.046.2315112
GCGTCTA5400.046.1107142
GGGTCTA23600.046.1053622
GGCCATA31900.044.88613510
GAACGCG31800.044.3823524
TGAACGC31950.044.13910323
CGGTCTA3950.044.0398522
ATCTCGT32250.043.97618535
TACCACC32550.043.67492715
Produced by FastQC (version 0.11.5)