FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318042

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318042
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3393279
Sequences flagged as poor quality0
Sequence length35-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN133840.39442674769743363No Hit
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG130820.3855268016570403No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG51780.15259576356674473No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC47500.13998259500618723No Hit
GGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTC41520.1223595230454083No Hit
CGCTCCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTA35360.1042059907246059No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34650.10211361930451343No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG34520.10173050904449649No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCCGT15500.037.9104470
CGCGGCA38350.036.24421732
GCGGCAC41000.034.0147533
TCGCCGG39850.033.7919348
CCCGTCG41150.032.9224544
CCGTCGC41100.032.56289745
CGGCACC43700.031.93324734
GTTATCG2150.031.7133736
ATGGCCG14800.031.07680369
TAGTCCG2450.030.6131136
CCCCCGA46750.030.03256219
TTATCGG2400.029.830397
ACCCGCG47500.029.81714625
GCACCCC47950.029.52059736
CGCCCTA8000.028.64378761
CACCCCC50050.028.24421537
GTCGCCT3300.027.02760170
CCCCGAC51700.026.97407520
GGCACCC53200.026.44826735
CCGCGCG54300.026.24656327