FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318046

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318046
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3510342
Sequences flagged as poor quality0
Sequence length35-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG150940.4299865938988281No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG52870.15061210560110666No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG40100.11423388376403211No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA41400.037.54413232
GCGGCAC43400.035.81859633
TCGCCGG42700.034.10435548
CGGCACC46200.033.7301134
CCGTCGC44600.032.92944345
CCCGTCG44750.032.8836444
ACCCGCG50000.031.52857425
CCCCCGA50650.031.30773219
CCCCGAC52900.029.91621420
GCACCCC54550.029.08432436
AGTCCGC55050.029.0482657
CCGCGCG54800.028.77721227
CAGTCCG52150.028.539726
CACCCCC55950.028.17565537
TCCGCCC58400.027.7375819
TAGTCCG4250.027.6901666
CCCGACC57450.027.54844521
GTCCGCC58150.027.3806468
CCCCCGT53750.027.35872842
GTGCCGT7400.027.2212281
Produced by FastQC (version 0.11.5)