FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318047

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318047
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3510342
Sequences flagged as poor quality0
Sequence length35-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC52550.14970051351121916No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC48710.13876140843256868No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGGT32850.047.8244139
TGGCGCG33250.046.72605515
CGCGGTG38600.041.14855610
CTACTCG39350.039.69846737
TACTCGG40350.038.7223738
AGGATCG41100.037.1165959
GTAGTCC42100.036.954727
CGGCATA3900.036.540831
ACTCGGG43100.036.34160639
TAGTCCC43050.036.22331228
GGGGTAG18350.035.9895741
GATCGCT42400.035.6865761
GCGGTAT2450.035.467711
TGTAGTC44350.035.30872326
CGTGCCT43700.035.09135420
CGGGTAG5850.034.4612731
CCGAACT5850.034.4447567
GGGGGGA69150.033.727921
GCGTGCC46250.033.5260319
GGGGTAT14300.033.299961
Produced by FastQC (version 0.11.5)