FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318048

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318048
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3502096
Sequences flagged as poor quality0
Sequence length35-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG149570.4270870929866001No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG54140.15459313508253342No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG38590.11019115409743192No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA44250.036.141232
GCGGCAC46250.034.73481833
TCGCCGG44950.034.48178548
CCGTCGC46450.033.55897545
CCCGTCG46650.033.55415344
CGGCACC48600.032.84671834
CCCCCGA52250.030.61790519
ACCCGCG53500.029.72577725
GCACCCC55950.028.91796736
CCCCGAC55600.028.8395220
CACCCCC56650.028.5071137
GTTCCGT2700.028.4478631
CCGCGCG57050.028.00793527
AGTCCGC58000.027.930747
CGTACGG755.8418565E-527.82039343
CAGTCCG56150.027.5563936
CCCCCGT56350.027.51548242
TCCGCCC61000.027.0110689
GCGCGGG57550.026.86130762
GGCACCC59850.026.79578235
Produced by FastQC (version 0.11.5)